Robert Koch

Robert Koch (born Dec. 11, 1843, Clausthal, Hannover [now Clausthal-Zellerfeld, Ger.]—died May 27, 1910, Baden-Baden, Ger.) was a German physician and one of the founders of bacteriology. He discovered the anthrax disease cycle (1876) and the bacteria responsible for tuberculosis (1882) and cholera (1883). For his discoveries in regard to tuberculosis, he received the Nobel Prize for Physiology or Medicine in 1905.

Early training

Koch attended the University of Göttingen, where he studied medicine, graduating in 1866. He then became a physician in various provincial towns. After serving briefly as a field surgeon during the Franco-Prussian War of 1870–71, he became district surgeon in Wollstein, where he built a small laboratory. Equipped with a microscope, a microtome (an instrument for cutting thin slices of tissue), and a homemade incubator, he began his study of algae, switching later to pathogenic (disease-causing) organisms.

Anthrax research

One of Koch’s teachers at Göttingen was anatomist and histologist Friedrich Gustav Jacob Henle, who in 1840 had published the theory that infectious diseases are caused by living microscopic organisms. In 1850 French parasitologist Casimir Joseph Davaine was among the first to observe organisms in the blood of diseased animals. In 1863 he reported the transmission of anthrax by the inoculation of healthy sheep with the blood of animals dying of the disease and the finding of microscopic rod-shaped bodies in the blood of both groups of sheep. Inspired by the work of French microbiologist Louis Pasteur, Davaine showed that it was highly probable that, because the sheep did not become diseased in the absence of these rodlike bodies, anthrax was due to the presence of such organisms in the blood. The natural history of the disease was, nevertheless, far from complete.

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It was at that point that Koch began his investigation of anthrax. Through field observations he noticed that the disease would reappear in grazing animals even after long periods with no apparent source of infection. In a series of laboratory experiments, he cultivated the anthrax organisms in suitable media on microscope slides, demonstrated their growth into long filaments, and discovered the formation within them of oval, translucent bodies—dormant spores. Koch found that the dried spores could remain viable for years, even under exposed conditions. The finding explained the recurrence of the disease in pastures long unused for grazing, for the dormant spores could, under the right conditions, develop into the rod-shaped bacteria (bacilli) that cause anthrax. The anthrax life cycle, which Koch had discovered, was announced and illustrated at Breslau in 1876, on the invitation of Ferdinand Cohn, an eminent botanist. Julius Cohnheim, a famous pathologist, was deeply impressed by Koch’s presentation. “It leaves nothing more to be proved,” he said.

I regard it as the greatest discovery ever made with bacteria and I believe that this is not the last time that this young Robert Koch will surprise and shame us by the brilliance of his investigations.

Cohn, whose discovery of spores had been published in 1875, was also very much impressed and generously helped to prepare the engraving for Koch’s epochal paper, which he also published. One of Cohn’s pupils, Joseph Schroeter, found that chromogenic (color-forming) bacteria would grow on such solid substrates as potato, coagulated egg white, meat, and bread and that those colonies were capable of forming new colonies of the same color, consisting of organisms of the same type. That was the starting point of Koch’s pure-culture techniques, which he worked out a few years later. That a disease organism might be cultured outside the body was a concept introduced by Pasteur, but the pure-culture techniques for doing so were perfected by Koch, whose precise and ingenious experiments demonstrated the complete life cycle of an important organism. The anthrax work afforded for the first time convincing proof of the definite causal relation of a particular microorganism to a particular disease.

Contributions to general bacteriology and pathology

In 1877 Koch published an important paper on the investigation, preservation, and photographing of bacteria. His work was illustrated by superb photomicrographs. In his paper he described his method of preparing thin layers of bacteria on glass slides and fixing them by gentle heat. Koch also invented the apparatus and the procedure for the very useful hanging-drop technique, whereby microorganisms could be cultured in a drop of nutrient solution on the underside of a glass slide. The technique allowed microorganisms to be observed alive and undisturbed in a small drop of liquid, making it possible to study their movement, growth, and behavior.

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In 1878 Koch summarized his experiments on the etiology of wound infection. By inoculating animals with material from various sources, he produced six types of infection, each caused by a specific microorganism. He then transferred these infections by inoculation through several kinds of animals, reproducing the original six types. In that study, he observed differences in pathogenicity for different species of hosts and demonstrated that the animal body is an excellent apparatus for the cultivation of bacteria.

Koch, now recognized as a scientific investigator of the first rank, obtained a position in Berlin in the Imperial Health Office, where he set up a laboratory in bacteriology. With his collaborators, he devised new research methods to isolate pathogenic bacteria. Koch determined guidelines to prove that a disease is caused by a specific organism. These four basic criteria, called Koch’s postulates, are:

1. A specific microorganism is always associated with a specific disease.
2. The microorganism can be isolated from the diseased animal and grown in pure culture in the laboratory.
3. The cultured microorganism will cause the same disease when transferred to a healthy animal.
4. The microorganism can be isolated from the newly infected animal and confirmed as being identical to the original causative organism.