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recombinant DNA
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DNA extraction and recombinant DNA technology A step-by-step diagram shows donor DNA and vector DNA cut with restriction enzymes to create overhangs, mixed and sealed with ligase to form recombinant DNA, which is introduced into bacterial cells. The recombinant DNA replicates as the cells divide, producing clones. A step-by-step diagram illustrating the creation of a genomic DNA library: DNA is isolated from cells, cut with restriction enzymes, joined to plasmid vectors using DNA ligase, inserted into bacteria, and then amplified and collected for genetic research. Diagram illustrating the creation of a cDNA library: mRNA undergoes reverse transcription to form cDNA, which is cleaved and combined with cleaved plasmid vectors using ligation, then inserted into E. coli bacteria for amplification, resulting in test tubes containing a cDNA library of actively transcribed genes.
Learn how a genetic fingerprint is made using agarose gel, Southern blotting, and a radioactive DNA probe
A step-by-step diagram shows gene knockout in mice, starting with gene inactivation using recombinant DNA, incorporation into embryonic stem cells, injection into embryos, implantation in a female mouse, and resulting offspring with different genetic types for research. A step-by-step diagram shows the process of creating genetically modified plants using recombinant DNA technology, from inserting an insecticidal gene into plant cells to growing plants that kill insect pests. Dense rows of tall green corn plants in a genetically modified corn field, with tassels visible at the top against a pale sky. Microarray
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recombinant DNA technology summary
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recombinant DNA: References & Edit History

Article History

Type Description Contributor Date
Modified link of Web site: Biology LibreTexts - Recombinant DNA Technology. Oct 01, 2026
Top question added. May 14, 2026
Add new Web site: Biological Principles - Recombinant DNA. Dec 19, 2025
Add new Web site: University of Baghdad - Institute of Genetic Engineering and Biotechnology - Recombinant DNA Technology (PDF). Mar 31, 2025
Questions and answers added. Feb 11, 2025
Add new Web site: International Journal of Advanced Research in Biological Sciences - Application of Recombinant DNA technology in Agriculture: A Review (PDF). Jan 23, 2025
Cross references added. Sep 24, 2024
Add new Web site: International Journal of Pharmaceutical Sciences Review and Research - Recombinant DNA Technology: Applications in the Field of Biotechnology and Criome Sciences. Aug 30, 2024
Clarified opening sentence. Aug 27, 2024
Add new Web site: Journal of Emerging Technologies and Innovative Research - An overview on Recombinant DNA Technology and its applications. Jul 17, 2024
Add new Web site: Academia - The role of recombinant DNA technology for human welfare. Dec 13, 2023
Add new Web site: Iowa State University Digital Press - Recombinant DNA Technology. Oct 06, 2023
Add new Web site: Nature - Recombinant DNA Technology and Transgenic Animals. Jul 07, 2023
Add new Web site: Biology LibreTexts - Recombinant DNA Technology. Oct 26, 2022
Add new Web site: Biology LibreTexts - Recombinant DNA Technology. Oct 26, 2022
Changed “genetically modified organism” to “genetically engineered organism.” Oct 14, 2020
Changed article title from "recombinant DNA technology" to "recombinant DNA." Jun 05, 2019
Add new Web site: What Is Biotechnology? - Recombinant DNA. Dec 07, 2018
Add new Web site: University of Leicester - Recombinant DNA and Genetic Techniques. Dec 07, 2018
Added information on the invention of recombinant DNA technology. Oct 18, 2018
Add new Web site: National Center for Biotechnology Information - PubMed Central - Role of Recombinant DNA Technology to Improve Life. Oct 05, 2018
Corrected display issue. Jan 03, 2018
Add new Web site: Khan Academy - DNA cloning and recombinant DNA. Sep 05, 2016
Add new Web site: Rensselaer Polytechnic Institute - The Basics of Recombinant DNA. Apr 08, 2016
Add new Web site: MIT OpenCourseWare - Recombinant DNA. Apr 08, 2016
Added a photo of genetically modified corn (maize) and art depicting the generation of cDNA and genomic DNA libraries and the process of gene knockout. Aug 28, 2009
New article added. Sep 26, 2007
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