recombinant DNA: Quick Facts

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Also Known As	recombinant DNA technology

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DNA extraction and recombinant DNA technology

A step-by-step diagram shows donor DNA and vector DNA cut with restriction enzymes to create overhangs, mixed and sealed with ligase to form recombinant DNA, which is introduced into bacterial cells. The recombinant DNA replicates as the cells divide, producing clones.

A step-by-step diagram illustrating the creation of a genomic DNA library: DNA is isolated from cells, cut with restriction enzymes, joined to plasmid vectors using DNA ligase, inserted into bacteria, and then amplified and collected for genetic research.

Diagram illustrating the creation of a cDNA library: mRNA undergoes reverse transcription to form cDNA, which is cleaved and combined with cleaved plasmid vectors using ligation, then inserted into E. coli bacteria for amplification, resulting in test tubes containing a cDNA library of actively transcribed genes.

A step-by-step diagram shows gene knockout in mice, starting with gene inactivation using recombinant DNA, incorporation into embryonic stem cells, injection into embryos, implantation in a female mouse, and resulting offspring with different genetic types for research.

A step-by-step diagram shows the process of creating genetically modified plants using recombinant DNA technology, from inserting an insecticidal gene into plant cells to growing plants that kill insect pests.

Dense rows of tall green corn plants in a genetically modified corn field, with tassels visible at the top against a pale sky.

Learn how a genetic fingerprint is made using agarose gel, Southern blotting, and a radioactive DNA probe

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Topics

in vitro mutagenesis gene disruption DNA genetic engineering gene editing pharming genetically modified organism three-parent baby whole genome sequencing monoclonal antibody DNA sequencing

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Key People

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Stanley Cohen

American biochemist

Paul Berg

Paul Berg

American biochemist

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Mario R. Capecchi

American scientist