phage display
Quick Summary
Phage display is a laboratory technique developed by George P. Smith that uses bacteriophages to study protein interactions. Bacteriophages are viruses that infect bacteria. In phage display, foreign DNA fragments are fused into phage gene III, causing the resulting proteins to be displayed on the surface of the phage virion. This allows researchers to use antibodies to select for specific fusion phages, effectively amplifying foreign gene sequences for further study. This technique has been crucial in developing antibody-based therapies, such as Adalimumab (Humira), used to treat rheumatoid arthritis. For their work on phage display, Smith and Greg Winter were awarded the Nobel Prize in Chemistry in 2018.12
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- bacteriophageRole in laboratory research
… developed a technology known as phage display, which allowed for the generation of engineered proteins. Such proteins were produced by fusing foreign or engineered DNA fragments into phage gene III. Gene III encodes a protein expressed on the phage virion surface. Thus, gene III fusion proteins taken up by phages…
Read More… developed a technology known as phage display, which allowed for the generation of engineered proteins. Such proteins were produced by fusing foreign or engineered DNA fragments into phage gene III. Gene III encodes a protein expressed on the phage virion surface. Thus, gene III fusion proteins taken up by phages…
Read More - George P. Smith
…known for his development of phage display, a laboratory technique employing bacteriophages (bacteria-infecting viruses) for the investigation of protein-protein, protein-DNA, and protein-peptide interactions. Phage display proved valuable to the development of treatments for conditions such as
Read More…known for his development of phage display, a laboratory technique employing bacteriophages (bacteria-infecting viruses) for the investigation of protein-protein, protein-DNA, and protein-peptide interactions. Phage display proved valuable to the development of treatments for conditions such as
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