ultraviolet-visible spectrophotometry

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July 14, 2026

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Ultraviolet-visible (UV-visible) spectrophotometry is a technique used in chemical analysis to measure how much electromagnetic radiation a substance absorbs. This method is particularly useful for organic compounds containing pi bonds, as these bonds can be excited by UV-visible light. The amount of light absorbed is related to the concentration of the substance and the path length of the light through the sample, as described by Beer's law. By analyzing the wavelengths of light absorbed, scientists can gain information about a molecule's structure and determine its concentration in a solution. This technique is valuable for both qualitative and quantitative analysis.123

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chemical analysis
Ultraviolet-visible spectrophotometry
Absorption in the ultraviolet-visible region of the spectrum causes electrons in the outermost occupied orbital of an atom or molecule to be moved to a higher (i.e., farther from the nucleus) unoccupied orbital. Ultraviolet-visible absorptiometry is principally used for quantitative analysis of atoms…
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Absorption in the ultraviolet-visible region of the spectrum causes electrons in the outermost occupied orbital of an atom or molecule to be moved to a higher (i.e., farther from the nucleus) unoccupied orbital. Ultraviolet-visible absorptiometry is principally used for quantitative analysis of atoms…
Read More