stable transfection

Stable transfection is a biological technique used to introduce foreign nucleic acid (like DNA or RNA) into a cell. Unlike transient transfection, where the introduced genetic material is temporary, stable transfection results in the integration of the nucleic acid into the cell's genome. This allows for long-term expression of the foreign genetic information and enables researchers to study its effects over extended periods. Various methods, including physical (e.g., electroporation) and chemical (e.g., using lipids or polymers) approaches, can be employed to achieve stable transfection. This technique is valuable in fields such as cancer research and drug discovery.1