retardation factor
Quick Summary
The retardation factor, often denoted as Rf, is a value used in chromatography to describe how far a substance has moved relative to the solvent front. It is calculated by dividing the distance the center of a solute zone has traveled from the origin by the distance the mobile phase front has traveled from the origin. This value is constant for a given solute under specific solvent conditions and helps in qualitative analysis by comparing the Rf of unknown components to known substances. In planar chromatography, the Rf value is a key measurement reported for separated zones.12
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chemical analysisLiquid chromatography…is performed by comparing the retardation factor (Rf) of the analyte components with the retardation factors of known substances. The retardation factor is defined as the distance from the original sample spot that the component has moved divided by the distance that the mobile phase front has moved and is…
Read More…is performed by comparing the retardation factor (Rf) of the analyte components with the retardation factors of known substances. The retardation factor is defined as the distance from the original sample spot that the component has moved divided by the distance that the mobile phase front has moved and is…
Read More- chromatographyDevelopment chromatography
…reported in terms of the Rf value, the distance moved by the centre of the zone relative to the distance moved by the mobile phase front, where both are measured from the origin. Use of the solvent front as a reference point is frequently inconvenient. A standard solute is often…
Read More…reported in terms of the Rf value, the distance moved by the centre of the zone relative to the distance moved by the mobile phase front, where both are measured from the origin. Use of the solvent front as a reference point is frequently inconvenient. A standard solute is often…
Read More