peak
Quick Summary
In chromatography, a chromatogram is a visual representation of a separation. It can be a pattern formed on a medium or a time-based graphic record. In elution chromatography, a detector monitors the emerging mobile phase, and its signal is recorded as a peak on a chart recorder or stored digitally.12
Ideally, these peaks follow a Gaussian distribution. The peak maximum indicates the point of highest concentration, and the peak width is crucial for determining how well different components are separated (resolution). The time it takes for a solute to elute from the column, known as retention time, is a key characteristic used to identify substances.234
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- chromatographyElution chromatography
…which is registered as a peak on a strip-chart recorder. The recorder trace where solute is absent is the baseline. A plot of the solute concentration along the migration coordinate of development chromatograms yields a similar solute peak. Collectively the plots are the concentration profiles; ideally they are Gaussian (normal,…
Read More…which is registered as a peak on a strip-chart recorder. The recorder trace where solute is absent is the baseline. A plot of the solute concentration along the migration coordinate of development chromatograms yields a similar solute peak. Collectively the plots are the concentration profiles; ideally they are Gaussian (normal,…
Read More - chromatographyChromatography–mass spectrometry methods
…drift, start integration when a peak appears, integrate, and stop the process when the peak exits the detector. Integration, a process of summation, is accomplished by opening and closing a narrow electronic window, registering the signal intensity, repeating the process, and then summing the stored signals to produce a number…
Read More…drift, start integration when a peak appears, integrate, and stop the process when the peak exits the detector. Integration, a process of summation, is accomplished by opening and closing a narrow electronic window, registering the signal intensity, repeating the process, and then summing the stored signals to produce a number…
Read More