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A microbiological assay is a method used to determine the amount of B vitamins in a food sample. This technique works by measuring the growth rate of a specific microorganism that requires a particular vitamin. The microorganism is grown in a medium with known amounts of the vitamin, and its growth response is compared to that of a pure vitamin sample. This allows researchers to estimate the vitamin's availability in the food for the microorganism. This method is exclusively applicable to B vitamins.1

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