liquid chromatography
Quick Summary
Liquid chromatography (LC) is a separation technique in chemistry that uses a liquid mobile phase to move a sample through a stationary phase, typically packed in a column. Components of the sample separate based on their differing affinities for the stationary and mobile phases, causing them to travel through the column at different rates.123
LC is versatile and can separate a wide range of substances, including inorganic ions, organic molecules, and even large biological molecules like proteins. It is particularly useful for compounds that cannot be vaporized, unlike gas chromatography. High-performance liquid chromatography (HPLC) is a common and advanced form of LC that uses small particles and high pressure for efficient and rapid separations.1425
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chemical analysisChromatography…a liquid, the technique is liquid chromatography; if it is a gas, the technique is gas chromatography.
Read More…a liquid, the technique is liquid chromatography; if it is a gas, the technique is gas chromatography.
Read More- chemical analysisLiquid chromatography
This procedure can be performed either in a column or on a plane. Columnar liquid chromatography is used for qualitative and quantitative analysis in a manner similar to the way in which gas chromatography is employed. Sometimes retention volumes, rather than retention times,…
Read MoreThis procedure can be performed either in a column or on a plane. Columnar liquid chromatography is used for qualitative and quantitative analysis in a manner similar to the way in which gas chromatography is employed. Sometimes retention volumes, rather than retention times,…
Read More - separation and purificationChromatography
Since the early 1970s, liquid chromatography has developed as the premier separation method for organic substances. Because the mobile phase is a liquid, the requirement for vaporization is eliminated, and therefore LC can separate a much broader range of substances than GC. Species that have been successfully resolved include…
Read MoreSince the early 1970s, liquid chromatography has developed as the premier separation method for organic substances. Because the mobile phase is a liquid, the requirement for vaporization is eliminated, and therefore LC can separate a much broader range of substances than GC. Species that have been successfully resolved include…
Read More
- chromatographyLiquid chromatography
This form of chromatography employs a liquid mobile phase. Liquid-solid chromatography utilizes a solid stationary phase, and the major mechanism of retention is adsorption. Popular adsorbents are silica and alumina, which both retain polar compounds. If a polar mobile phase is used, the…
Read MoreThis form of chromatography employs a liquid mobile phase. Liquid-solid chromatography utilizes a solid stationary phase, and the major mechanism of retention is adsorption. Popular adsorbents are silica and alumina, which both retain polar compounds. If a polar mobile phase is used, the…
Read More - chromatographyLiquid chromatographic detectors
Liquid chromatographic detectors suitable for high-performance columns require clever technology. If the solutes contain structural features that absorb light at certain wavelengths, the decrease in the intensity of the transmitted beam of light compared to the intensity of the incident beam can…
Read MoreLiquid chromatographic detectors suitable for high-performance columns require clever technology. If the solutes contain structural features that absorb light at certain wavelengths, the decrease in the intensity of the transmitted beam of light compared to the intensity of the incident beam can…
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