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Ecological isolation is a type of reproductive isolating mechanism (RIM) that prevents different populations from interbreeding. This occurs when populations live in the same geographic area but occupy different habitats, thus rarely encountering each other.

For example, certain species of Anopheles mosquitoes, which are involved in malaria transmission, are reproductively isolated due to their breeding preferences. Some breed in brackish water, while others prefer stagnant or running fresh water. This difference in habitat, even within the same territory, acts as a barrier to interbreeding.1

Ecological isolation is one of several barriers that can lead to the differentiation of biological units, such as species.23

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