continuous-flow paper electrophoresis

Continuous-flow paper electrophoresis is a method used for separating substances based on their electrical charge and mobility. In this technique, a sample is continuously introduced at the top of a vertical sheet of paper. As the sample flows downward, it is subjected to an electrical potential applied perpendicular to the direction of flow.1

Different components of the sample will disperse across the paper according to their charge and how quickly they move in the electric field. These separated components then drop from the bottom edge of the paper into separate collection vessels. This method is a form of electrophoresis, which is the movement of charged particles in a fluid under the influence of an electric field.12