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amplification
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DNA sequencing
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amplification

genetics
Also known as: gene amplification
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DNA sequencing
DNA sequencing In genomics research, fragments of genomic DNA are inserted into a vector and amplified by replication in bacterial cells. In this way, large amounts of DNA can be cloned and extracted from the bacterial cells. The DNA is then sequenced and further analyzed using bioinformatics techniques.

Gene amplification is a chromosomal abnormality where the number of copies of a proto-oncogene increases, leading to excessive protein production. This can contribute to cancer development. For instance, amplification of the N-MYC proto-oncogene is found in about 40% of neuroblastoma cases, with a higher copy number correlating to more advanced disease. Similarly, amplification of the c-ERBB2 (HER2) proto-oncogene occurs in some breast cancers.1

While not directly related to gene amplification in cancer, the polymerase chain reaction (PCR) is a technique that makes many copies of a specific DNA segment, crucial for various biological experiments and diagnostics.2

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2 Britannica Sources
  1. cancer - Gene amplification
  2. polymerase chain reaction

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