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Which cells in the testes produce testosterone?

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In the human testes, testosterone is produced by Leydig (interstitial) cells.1

These cells are located in the spaces between the seminiferous tubules, as well as beneath the tunica albuginea and within the septal walls of the testes. Their activity is regulated by luteinizing hormone, which is secreted by the anterior pituitary gland and binds to receptors on the surface of the Leydig cells to stimulate hormone production.1