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Azotobacter are a genus of large, rod-shaped or spherical bacteria found in soil and sewage. These bacteria are significant because they are capable of fixing atmospheric nitrogen, converting it into a form usable by plants. This process is crucial for the nitrogen cycle, as nitrogen is essential for plant growth. Azotobacter are classified as free-living, or nonsymbiotic, nitrogen-fixing bacteria, distinguishing them from symbiotic bacteria like Rhizobium that associate with specific plants.12

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