How did Florence become part of the Grand Duchy of Tuscany?

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Florence became the center of the Grand Duchy of Tuscany through the consolidation of power by Cosimo I de' Medici during the 16th century.12

Following the assassination of Duke Alessandro de' Medici in 1537, Cosimo I was elected head of the Florentine government with the approval of the Holy Roman emperor Charles V. Cosimo I subsequently pursued a policy of territorial expansion, most notably launching a successful military campaign against the republic of Siena. By 1557, he had annexed Siena, bringing nearly all of Tuscany under his control.23

In 1569, Pope Pius V officially conferred the title of grand duke of Tuscany upon Cosimo I, formalizing the transition from a city-state ruled by a duke to a grand duchy. This established a new, authoritarian dynasty that governed Florence and the surrounding region until the Medici family line ended in 1737.2314