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The mezzo-soprano clef is a specific placement of the C clef on a musical staff. This clef indicates that middle C is located on the second line from the bottom of the staff.1

A clef is a symbol at the beginning of a musical staff that establishes the pitch of each line and space, serving as a reference for all notes. The three main clefs used today are the treble, bass, and C clefs, which are stylized versions of the letters G, F, and C, respectively.2

The mezzo-soprano voice range falls between the soprano and alto ranges, typically from the A below middle C to the second F or G above middle C.3

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