Which colleges played the first five-on-five college basketball game?

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The first college basketball game played with five players on each side took place between the University of Chicago and the University of Iowa.12

This historic match was held in Iowa City on January 18, 1896. The University of Chicago won the contest with a final score of 15–12, and notably, neither team utilized any substitutes during the game. H.F. Kallenberg, who had introduced the sport to his students at Iowa after learning the rules from the game's inventor, James Naismith, served as the referee.12