Why do researchers authenticate cultured cell lines?

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tissue culture Tissue culture often requires sterile working conditions and thus is typically performed in a laminar flow cabinet (or tissue culture hood), which circulates filtered air to reduce the risk of culture contamination.

Researchers authenticate cultured cell lines to ensure the accuracy and reproducibility of scientific experiments. Because cells in established lines accumulate mutations over time that can alter their character, it is essential for researchers across different laboratories to confirm they are working with the same biological material when comparing experimental results.1

Authentication is the process of verifying cell identity by comparing the DNA profile of the cultured cells against the known or standard profile for that specific cell line.1