Why do CAM plants open their stomata at night?

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CAM plants open their stomata at night primarily to conserve water in arid environments. By keeping their stomata closed during the hot daylight hours, these plants significantly reduce water loss through transpiration.12

Because the air is cooler and more humid at night, it is an ideal time for these plants to assimilate carbon dioxide without risking excessive dehydration. During these nocturnal hours, CAM plants fix carbon dioxide into organic acids, such as malate, which are then stored in the vacuoles of their cells.12

During the day, the stomata remain closed, and the stored malate is released and decarboxylated to provide a high concentration of carbon dioxide for the Calvin-Benson cycle. This temporal separation of carbon fixation stages allows CAM plants to thrive in environments where water is scarce.12