How do plants use carbon dioxide during photosynthesis?

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Plants use carbon dioxide (CO2) during the "dark reactions" of photosynthesis, a process known as carbon fixation. In this stage, plants utilize the chemical energy (ATP and NADPH) generated during the light-harvesting reactions to convert inorganic carbon into organic compounds, such as sugars.12

The primary mechanism for this conversion is the Calvin-Benson cycle, where the enzyme Rubisco catalyzes the addition of CO2 to a five-carbon sugar called ribulose 1,5-bisphosphate (RuBP). This reaction produces molecules that are eventually synthesized into glucose.31

Plants have evolved different strategies to manage this process:
* C3 plants: Fix CO2 directly into RuBP using Rubisco.4
* C4 plants: Use an additional, spatially separated pathway to concentrate CO2 before it enters the Calvin-Benson cycle, which helps minimize photorespiration in warm or dry environments.45
* CAM plants: A variant of the C4 pathway where plants fix CO2 at night to prevent water loss during the day.4