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How did Wilhelm Roux maintain chick embryo tissue in his experiment?

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Tissue culture often requires sterile working conditions and thus is typically performed in a laminar flow cabinet (or tissue culture hood), which circulates filtered air to reduce the risk of culture contamination.
tissue culture Tissue culture often requires sterile working conditions and thus is typically performed in a laminar flow cabinet (or tissue culture hood), which circulates filtered air to reduce the risk of culture contamination.

In his 1885 experiment, German zoologist Wilhelm Roux maintained chick embryo tissue by cultivating it in a warm salt solution. This work is recognized as an early attempt at tissue culture, a method of biological research where tissue fragments are transferred to an artificial environment to survive and function outside of the organism.1