A dihybrid testcross is used to determine an organismās genotype for two traits. It involves crossing an organism with an unknown genotype for the two traits under investigation with an organism that is homozygous recessive for both traits. In addition to the organismās genotype, this type of cross reveals gene linkages, if any. If the dominant organism is heterozygous for the genes of interest and the genes assort independently, then the resulting offspring display a phenotypic ratio of 1:1:1:1.
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