Catalase activity is influenced by several factors that affect how well the enzyme functions. Temperature and pH are critical, as extreme conditions can alter the enzyme’s structure and reduce its effectiveness. The rate of reaction also depends on the concentrations of both the enzyme and its substrate, hydrogen peroxide, wherein reaction rate increases until the enzyme becomes saturated. In addition, certain chemicals can inhibit catalase activity, and in whole tissues, greater surface area can expose more enzyme and accelerate the reaction.
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