Restriction enzyme: Quick Facts

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Facts

Also Known As	restriction endonuclease

Photos

Diagram illustrating the creation of a cDNA library: mRNA undergoes reverse transcription to form cDNA, which is cleaved and combined with cleaved plasmid vectors using ligation, then inserted into E. coli bacteria for amplification, resulting in test tubes containing a cDNA library of actively transcribed genes.

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Topics

type II restriction enzyme type IV restriction enzyme type III restriction enzyme type I restriction enzyme Nuclease

Key People

Smith, Hamilton O.

Hamilton O. Smith

American biologist

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Werner Arber

Swiss microbiologist