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What interval between the first and third notes distinguishes a minor scale from a major scale?

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The interval between the first and third notes is the primary factor that distinguishes a major scale from a minor scale. In a major scale, this interval is a major third, while in a minor scale, the third note is lowered by a half step, resulting in a minor third.12

Specifically, the major scale is defined by its specific pattern of intervals, where the third tone is a major third above the tonic. In contrast, the minor scale—including the natural, harmonic, and melodic forms—is characterized by a lowered third tone, creating a minor third interval from the first note.12