Are mountain lions closely related to African lions?

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Mountain lions are not closely related to African lions in terms of their biological classification. While both are members of the family Felidae, they belong to different subfamilies and genera.12

African lions are classified in the genus Panthera, which consists of the "big" or "roaring" cats, including tigers, leopards, and jaguars. In contrast, the mountain lion—also known as the puma or cougar—is a New World member of the genus Puma and belongs to the subfamily Felinae.12

Because they belong to distinct evolutionary lineages within the cat family, they are not considered close relatives.12